Eight suicide smokers had high levels of nicotine and cotinine in their blood (mean=115 ng/ml, SD=49, and mean=405 ng/ml, SD=291, respectively) and urine (mean=1940 ng/ml, SD=2540, and mean=1170 ng/ml, SD=1570, respectively). In contrast, eight nonsuicide smokers had lower levels of nicotine and cotinine in their blood (mean=30.1 ng/ml, SD=17.7, and mean=122 ng/ml, SD=65, respectively) and urine (mean=383 ng/ml, SD=417, and mean=170 ng/ml, SD=86, respectively). Blood nicotine levels in our eight suicide smokers were significantly higher than in nonsuicide smokers (t=4.61, df=14, p=0.0004). Although urine nicotine level and blood and urine cotinine levels were higher in suicide than nonsuicide smokers, they did not achieve a level of statistical significance. In six of 20 nonsmokers, nicotine and/or cotinine originating from passive smoking were detected. Neither nicotine nor cotinine was detected in the remaining 14 cases.